Production of an extracellular chitinolytic system byTalaromyces emersonii CBS 814.70.

L. Hendy; J. Gallagher; A. Winters; T. J. Hackett; L. McHale; A. P. McHale

doi:10.1007/BF01088192

Production of an extracellular chitinolytic system byTalaromyces emersonii CBS 814.70.

L. Hendy, J. Gallagher, A. Winters, T. J. Hackett, L. McHale, A. P. McHale

Department of Life Sciences

Trinity College Dublin

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The thermophilic fungusTalaromyces emersonii CBS 814.70 produces a thermostable extracellular chitinolytic system when cultured on chitin containing media. The chitinolytic system consists of chitinase (EC 3.2.1.14) and N-acetylglucosaminidase (EC 3.2.1.30). Using fluorescent substrate analogues, in zymogram staining of polyacrylamide gradient and isoelectric focusing gels on which the chitinase system was electrophoresed and focused, respectively, it was found that a number of bands could be resolved. Using isoelectric focusing it was observed that at least 4 extracellular forms of chitinase activity are produced.

Original language	English
Pages (from-to)	673-678
Number of pages	6
Journal	Biotechnology Letters
Volume	12
Issue number	9
DOIs	https://doi.org/10.1007/BF01088192
Publication status	Published - Sept 1990

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title = "Production of an extracellular chitinolytic system byTalaromyces emersonii CBS 814.70.",

abstract = "The thermophilic fungusTalaromyces emersonii CBS 814.70 produces a thermostable extracellular chitinolytic system when cultured on chitin containing media. The chitinolytic system consists of chitinase (EC 3.2.1.14) and N-acetylglucosaminidase (EC 3.2.1.30). Using fluorescent substrate analogues, in zymogram staining of polyacrylamide gradient and isoelectric focusing gels on which the chitinase system was electrophoresed and focused, respectively, it was found that a number of bands could be resolved. Using isoelectric focusing it was observed that at least 4 extracellular forms of chitinase activity are produced.",

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AU - Hendy, L.

AU - Gallagher, J.

AU - Winters, A.

AU - Hackett, T. J.

AU - McHale, L.

AU - McHale, A. P.

PY - 1990/9

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AB - The thermophilic fungusTalaromyces emersonii CBS 814.70 produces a thermostable extracellular chitinolytic system when cultured on chitin containing media. The chitinolytic system consists of chitinase (EC 3.2.1.14) and N-acetylglucosaminidase (EC 3.2.1.30). Using fluorescent substrate analogues, in zymogram staining of polyacrylamide gradient and isoelectric focusing gels on which the chitinase system was electrophoresed and focused, respectively, it was found that a number of bands could be resolved. Using isoelectric focusing it was observed that at least 4 extracellular forms of chitinase activity are produced.

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DO - 10.1007/BF01088192

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VL - 12

SP - 673

EP - 678

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 9

ER -

Production of an extracellular chitinolytic system byTalaromyces emersonii CBS 814.70.

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