Introgression mapping in the grasses. I: Introgression of Festuca pratensis chromosomes and chromosome segments into Lolium perenne

I. P. King; W. G. Morgan; I. P. Armstead; J. A. Harper; M. D. Hayward; A. Bollard; J. V. Nash; J. W. Forster; H M Thomas

doi:10.1046/j.1365-2540.1998.00437.x

Introgression mapping in the grasses. I: Introgression of Festuca pratensis chromosomes and chromosome segments into Lolium perenne

I. P. King, W. G. Morgan, I. P. Armstead, J. A. Harper, M. D. Hayward, A. Bollard, J. V. Nash, J. W. Forster, H M Thomas

Department of Life Sciences

Institute of Grassland and Environmental Research

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

Lolium perenne (4x)/Festuca pratensis (2x) triploid hybrids :(2n = 3x = 21) were produced and backcrossed to Lolium perenne. The BC ₁ progeny, which predominantly had 14 chromosomes, were analysed using genomic in situ hybridization (GISH) and genetic markers. GISH revealed that over 74% of the BC ₁ individuals carried one or more E pratensis chromosome segments. By comparing the physical size of introgressed F. pratensis chromosome segments with the presence or absence of F. pratensis-specific polymorphisms, it was possible to determine the physical position of genetic markers. The potential of a new type of genetic mapping ('introgression mapping') for the alignment of physical and genetic maps, determining the genetic control of agronomically important characters and the production of novel germplasm for the development of new varieties is discussed.

Original language	English
Pages (from-to)	462-467
Number of pages	6
Journal	Heredity
Volume	81
Issue number	4
DOIs	https://doi.org/10.1046/j.1365-2540.1998.00437.x
Publication status	Published - 01 Oct 1998

Keywords

Genetic mapping
Genomic in situ hybridization
Introgression mapping
Physical mapping
Recombination

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title = "Introgression mapping in the grasses. I: Introgression of Festuca pratensis chromosomes and chromosome segments into Lolium perenne",

abstract = "Lolium perenne (4x)/Festuca pratensis (2x) triploid hybrids :(2n = 3x = 21) were produced and backcrossed to Lolium perenne. The BC 1 progeny, which predominantly had 14 chromosomes, were analysed using genomic in situ hybridization (GISH) and genetic markers. GISH revealed that over 74% of the BC 1 individuals carried one or more E pratensis chromosome segments. By comparing the physical size of introgressed F. pratensis chromosome segments with the presence or absence of F. pratensis-specific polymorphisms, it was possible to determine the physical position of genetic markers. The potential of a new type of genetic mapping ('introgression mapping') for the alignment of physical and genetic maps, determining the genetic control of agronomically important characters and the production of novel germplasm for the development of new varieties is discussed. ",

keywords = "Genetic mapping, Genomic in situ hybridization, Introgression mapping, Physical mapping, Recombination",

author = "King, {I. P.} and Morgan, {W. G.} and Armstead, {I. P.} and Harper, {J. A.} and Hayward, {M. D.} and A. Bollard and Nash, {J. V.} and Forster, {J. W.} and Thomas, {H M}",

note = "Funding Information: I. P. Armstead and A. Bollard are funded by the Biotechnology and Biological Sciences Research Council{\textquoteright}s Plant and Animal Genome Analysis initiative.",

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doi = "10.1046/j.1365-2540.1998.00437.x",

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T2 - Introgression of Festuca pratensis chromosomes and chromosome segments into Lolium perenne

AU - King, I. P.

AU - Morgan, W. G.

AU - Armstead, I. P.

AU - Harper, J. A.

AU - Hayward, M. D.

AU - Bollard, A.

AU - Nash, J. V.

AU - Forster, J. W.

AU - Thomas, H M

N1 - Funding Information: I. P. Armstead and A. Bollard are funded by the Biotechnology and Biological Sciences Research Council’s Plant and Animal Genome Analysis initiative.

PY - 1998/10/1

Y1 - 1998/10/1

N2 - Lolium perenne (4x)/Festuca pratensis (2x) triploid hybrids :(2n = 3x = 21) were produced and backcrossed to Lolium perenne. The BC 1 progeny, which predominantly had 14 chromosomes, were analysed using genomic in situ hybridization (GISH) and genetic markers. GISH revealed that over 74% of the BC 1 individuals carried one or more E pratensis chromosome segments. By comparing the physical size of introgressed F. pratensis chromosome segments with the presence or absence of F. pratensis-specific polymorphisms, it was possible to determine the physical position of genetic markers. The potential of a new type of genetic mapping ('introgression mapping') for the alignment of physical and genetic maps, determining the genetic control of agronomically important characters and the production of novel germplasm for the development of new varieties is discussed.

AB - Lolium perenne (4x)/Festuca pratensis (2x) triploid hybrids :(2n = 3x = 21) were produced and backcrossed to Lolium perenne. The BC 1 progeny, which predominantly had 14 chromosomes, were analysed using genomic in situ hybridization (GISH) and genetic markers. GISH revealed that over 74% of the BC 1 individuals carried one or more E pratensis chromosome segments. By comparing the physical size of introgressed F. pratensis chromosome segments with the presence or absence of F. pratensis-specific polymorphisms, it was possible to determine the physical position of genetic markers. The potential of a new type of genetic mapping ('introgression mapping') for the alignment of physical and genetic maps, determining the genetic control of agronomically important characters and the production of novel germplasm for the development of new varieties is discussed.

KW - Genetic mapping

KW - Genomic in situ hybridization

KW - Introgression mapping

KW - Physical mapping

KW - Recombination

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DO - 10.1046/j.1365-2540.1998.00437.x

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JO - Heredity

JF - Heredity

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Introgression mapping in the grasses. I: Introgression of Festuca pratensis chromosomes and chromosome segments into Lolium perenne

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Keywords

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