TY - JOUR
T1 - In vitro biomarker discovery in the parasitic flatworm Fasciola hepatica for monitoring chemotherapeutic treatment
AU - Morphew, Russell Mark
AU - MacKintosh, Neil Douglas
AU - Hart, Elizabeth Helen
AU - Prescott, Mark
AU - LaCourse, E. James
AU - Brophy, Peter Michael
N1 - Morphew, R. M., MacKintosh, N. D., Hart, E. H., Prescott, M., LaCourse, E. J., Brophy, P. M. (2014). In vitro biomarker discovery in the parasitic flatworm Fasciola hepatica for monitoring chemotherapeutic treatment. EuPA Open Proteomics, 3, 85-99
PY - 2014/6
Y1 - 2014/6
N2 - The parasitic flatworm Fasciola hepatica is a global food security risk. With no vaccines, the sustainability of triclabendazole (TCBZ) is threatened by emerging resistance. F. hepatica excretory/secretory (ES) products can be detected in host faeces and used to estimate TCBZ success and failure. However, there are no faecal based molecular diagnostics dedicated to assessing drug failure or resistance to TCBZ in the field. Utilising in vitro maintenance and sub-proteomic approaches two TCBZ stress ES protein response fingerprints were identified: markers of non-killing and lethal doses. This study provides candidate protein/peptide biomarkers to validate for detection of TCBZ failure and resistance.
AB - The parasitic flatworm Fasciola hepatica is a global food security risk. With no vaccines, the sustainability of triclabendazole (TCBZ) is threatened by emerging resistance. F. hepatica excretory/secretory (ES) products can be detected in host faeces and used to estimate TCBZ success and failure. However, there are no faecal based molecular diagnostics dedicated to assessing drug failure or resistance to TCBZ in the field. Utilising in vitro maintenance and sub-proteomic approaches two TCBZ stress ES protein response fingerprints were identified: markers of non-killing and lethal doses. This study provides candidate protein/peptide biomarkers to validate for detection of TCBZ failure and resistance.
KW - Proteomics
KW - Biomarkers
KW - Triclabendazole
KW - Calreticulin
UR - http://hdl.handle.net/2160/13656
U2 - 10.1016/j.euprot.2014.02.013
DO - 10.1016/j.euprot.2014.02.013
M3 - Article
SN - 2212-9685
VL - 3
SP - 85
EP - 99
JO - EuPA Open Proteomics
JF - EuPA Open Proteomics
ER -
