TY - JOUR
T1 - An Analytical Pipeline for Quantitative Characterization of Dietary Intake
T2 - Application To Assess Grape Intake
AU - Garcia-Perez, Isabel
AU - Posma, Joram M
AU - Chambers, Edward S.
AU - Nicholson, Jeremy K.
AU - Mathers, John C.
AU - Beckmann, Manfred
AU - Draper, John
AU - Holmes, Elaine
AU - Frost, Gary
N1 - Funding Information:
IGP is supported by an NIHR postgraduate research fellowship, GF is supported by an NIHR senior investigator award. JCM, JD, MB, GF, and EH are supported by an MRC grant entitled Metabolomics for Monitoring Dietary Exposure (ref: MR/J010308/1). This study was supported by the Wellcome Trust NIHR Clinical Research Facility. The Section of Investigative Medicine is funded by grants from the MRC, BBSRC, NIHR, and an Integrative Mammalian Biology (IMB) Capacity Building Award. This article presents independent research funded by the National Institute for Health Research (NIHR). The views expressed are those of the authors and not necessarily those of the NHS, the NIHR or the Department of Health.
Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/3/23
Y1 - 2016/3/23
N2 - Lack of accurate dietary assessment in free-living populations requires discovery of new biomarkers reflecting food intake qualitatively and quantitatively to objectively evaluate effects of diet on health. We provide a proof-of-principle for an analytical pipeline to identify quantitative dietary biomarkers. Tartaric acid was identified by nuclear magnetic resonance spectroscopy as a dose-responsive urinary biomarker of grape intake and subsequently quantified in volunteers following a series of 4-day dietary interventions incorporating 0 g/day, 50 g/day, 100 g/day, and 150 g/day of grapes in standardized diets from a randomized controlled clinical trial. Most accurate quantitative predictions of grape intake were obtained in 24 h urine samples which have the strongest linear relationship between grape intake and tartaric acid excretion (r(2) = 0.90). This new methodological pipeline for estimating nutritional intake based on coupling dietary intake information and quantified nutritional biomarkers was developed and validated in a controlled dietary intervention study, showing that this approach can improve the accuracy of estimating nutritional intakes.
AB - Lack of accurate dietary assessment in free-living populations requires discovery of new biomarkers reflecting food intake qualitatively and quantitatively to objectively evaluate effects of diet on health. We provide a proof-of-principle for an analytical pipeline to identify quantitative dietary biomarkers. Tartaric acid was identified by nuclear magnetic resonance spectroscopy as a dose-responsive urinary biomarker of grape intake and subsequently quantified in volunteers following a series of 4-day dietary interventions incorporating 0 g/day, 50 g/day, 100 g/day, and 150 g/day of grapes in standardized diets from a randomized controlled clinical trial. Most accurate quantitative predictions of grape intake were obtained in 24 h urine samples which have the strongest linear relationship between grape intake and tartaric acid excretion (r(2) = 0.90). This new methodological pipeline for estimating nutritional intake based on coupling dietary intake information and quantified nutritional biomarkers was developed and validated in a controlled dietary intervention study, showing that this approach can improve the accuracy of estimating nutritional intakes.
KW - accurate dietary assessment
KW - metabolic profiling
KW - nutritional intake
KW - quantified dietary biomarkers
KW - tartaric acid
UR - http://hdl.handle.net/2160/42655
UR - http://www.scopus.com/inward/record.url?scp=84961898726&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.5b05878
DO - 10.1021/acs.jafc.5b05878
M3 - Article
C2 - 26909845
SN - 0021-8561
VL - 64
SP - 2423
EP - 2431
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 11
ER -
