Chemical modulation of Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) induces wide-scale biological and epigenomic changes - Supplementary tables and movies

Extended data This project contains the following extended data: Table S1. Chemical structures of the 39 compounds included in this study. Table S2. Z´ values for both phenotype and motility of the Roboworm screens performed on the 39 compounds. Table S3. Z´ values for both phenotype and motility of the Roboworm screens performed on the titration of the five selected compounds (compounds 15, 16, 33, 35 and 36). Table S4A. List of the 71,859 ATAC-Seq peaks found in the female dataset Table S4B. List of corresponding 844 genomic loci associated to statistically significant ATAC-seq differences (adjusted p-value ≤0.05) following compound 33 treatment (female dataset) Table S4C. List of the 84,997 ATAC-Seq peaks found in male dataset Table S4D. List of corresponding 2,107 genomic loci associated to statistically significant ATAC-seq differences (adjusted p-value ≤0.05) following compound 33 treatment (male dataset) Table S4E. List of female and male genes associated to statistically significant ATAC-seq differences between compound 33 and mock treatments. Table S5A. List of the 844 genomic loci associated to statistically significant ATAC-seq differences (female subset). The table contains the gene ID, chromosome position, start and end of the ATAC-seq peak position, ATAC-seq peak identifier, value of log2-foldchange (log2FC). Table S5B. Occurrence of ATAC-seq peaks (accessible chromatin) per genes (female subset). The table contains the gene ID and the associated number of ATAC-seq peaks Table S5C. List of ATAC-seq positive signals (accessible chromatin) found in the mock female samples (filtered from S5A Table with log2FC>0) Table S5D. List of ATAC-seq positive signals (accessible chromatin) found in the compound 33-treated female samples (filtered from S5A Table with log2FC0 corresponds to higher ATAC-seq positive signals found in the control treatment). Table S6A. List of corresponding 2,107 genomic loci associated with statistically significant ATAC-seq differences (male subset). The table contains the gene ID, chromosome position, start and end of the ATAC-seq peak position, ATAC-seq peak identifier, value of log2-foldchange (log2FC). Table S6B. Occurrence of ATAC-seq peaks per genes (male subset). The table contains the gene ID and the associated number of ATAC-seq peaks Table S6C. List of ATAC-seq positive signals (accessible chromatin) found in the mock male samples (filtered from S6A Table with log2FC>0) Table S6D. List of ATAC-seq positive signals (accessible chromatin) found in the compound 33-treated male samples (filtered from S6A Table with log2FC0 corresponds to higher ATAC-seq positive signals found in the control treatment). Table S7. List of the structure, the docking score and EC50 values (on schistosomula and adult worms) of the five most active compounds. Table S8. List of 24 adult worm ATAC-seq sample IDs, primer sequences and additional PCR cycles used for preparation of ATAC-seq libraries Table S9. Excel workbook used for the hypergeometric test for overrepresentation of ATAC-Seq signal within the available scRNA-Seq data derived from adult worms [70]. Movie S1. Video of S. mansoni compound 33-treated (3.13 µM, right-hand side) and control (DMSO, left-hand side) worm pairs after 72 h incubation in tissue culture wells. Notice the lack of parasite attachment and the presence of cellular material within the compound treated well. Movie S2. Serial optical sections of DAPI-stained, S. mansoni egg. Comparison between the compound 33-treated (3.13 µM, right-hand side) and the negative control (DMSO, left-hand side) egg is provided. Movie S3. Serial optical sections of S. mansoni adult female worm stained with DAPI and EdU. In these series of optical sections, three different anatomical regions (anterior region, gonadal system and posterior region, from left to right) of the worm were observed. Comparison between the negative control (DMSO, first row) and the compound 33-treated (3.13 µM, second row) parasites is provided. Movie S4. Serial optical sections of S. mansoni adult male worm stained with DAPI and EdU. In these series of optical sections, three different anatomical regions (anterior region, gonadal system and posterior region, from left to right) of the worm were observed. Comparison between the negative control (DMSO, first row) and the compound 33-treated (3.13 µM, second row) parasites is provided.